protogel83. Pour Stacking Gel Use ProtoGel Stacking Buffer to make 10 mL of a 4% stacking gel as follows: ProtoGel: 1.33 mL ProtoGel Stacking Buffer: 2.50 mL3. Pour Stacking Gel Use ProtoGel Stacking Buffer to make 10 mL of a 4% stacking gel as follows: ProtoGel: 1.33 mL ProtoGel Stacking Buffer: 2.50 mL